Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. 4. (VersaMax Molecular Devices, Sunnyvale, CA). Shark chondroitin sulfate A sodium salt (Sigma Aldrich Corp. 0 (Molecular Devices, Sunnyvale, CA). #0120-1293A SpectraMax, SoftMax Pro, Vmax and Emax are registered trademarks and VersaMax, Lmax,Although this manual has been prepared with every precaution to ensure accuracy, Molecular Devices Corporation assumes no liability for any errors or omissions, nor for any damages resulting from the application or use of this information. After incubation for 1 h, the medium was transferred to 96-well plates (SPL Life Sciences) in triplication and absorbance was measured by using the ELISA microplate reader (VersaMax, Molecular Devices, USA) at 490 nm. Molecular Devices Corporation. Molecular aspects of color variation in luciferase emission have been the subject of numerous studies: various mechanisms have been proposed [6] and multicolor reporter proteins have been created and applied in cell-based. The quantity of nitrite in the samples was calculated using the concentration of sodium nitrite as a standard. 0 OD. The assay was performed in 96-well microtiter plates in duplicate, and the absorbance was read at 405 nm with 620 nm as the reference wavelength using a microplate reader (VersaMax, Molecular Devices, Boston, Massachusetts). The emodin thermoreversible PG was. Molecular Devices Corporation SpectraTest ABS1 Validation Plate Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the operation of such Molecular Devices equipment, software, reagents, and. However, whether these cells exhibit the serotonergic neuronal phenotype observed in mature hippocampal neurons. We would like to show you a description here but the site won’t allow us. The optical density was measured at a wavelength of 450 nm using VersaMax (Molecular Devices, Seoul, South Korea). Get pricing and purchase lab equipment and. +49-89/96-05-88-0 fax +49-89/9-62-02-34-5 Japan Nihon Molecular Devices Osaka Tel. All samples were measured in triplicate. Although this manual has been prepared with every precaution to ensure accuracy, Molecular Devices SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. DMEM medium supplemented with the same volume of kit reagent on an empty well was used as a blank. To measure the luciferase activity biochemically, cells wereThe activity was measured colorimetrically at 450 nm using a microplate reader (Versamax, Molecular Devices, Sunnyvale, CA, USA). 1. 03 billion doses of. 00 USD Tecan Infinite F200 Reader Fluorescence Reader w WARRANTY $2,750. Our mission: find your user guide or user's manual as quickly as possible. However, whether these cells exhibit the serotonergic neuronal phenotype observed in mature hippocampal neurons. 4. 3860 N First Street San Jose,CA 95134 USA +1 408. This user guide provides detailed instructions on how to operate, maintain, and troubleshoot the device, as well as examples of applications and. 340‚ 850 nm. For validation of the Molecular Devices VersaMax™ Absorbance Microplate Reader for use with the Pyrochrome Assay, data acquisition and analysis were performed with SoftMax ® Pro GxP Software. Biosystems CytoFluor 4000 Multi Well Plate Reader $ 1,995. Readings were taken every 15 s at 490 nm and the results were analysed using SOFT-MaxPro 4. An aliquot of the samples dissolved in DMSO with 80 μL of phosphate buffer (pH 6. The SpectraTest® ABS1 Absorbance Validation. For pinocytosis, cells in wells were washed in PBS and 5 mg/ml of HPTS dye (in PBS) added and incubated with gentle rocking at 30 °C for 120 min. Molecular Devices公司的VersaMax读 板机为光栅型单色器读板机，拥有灵活检 测优势，无需加配额外的滤光片。 可调波长，无需滤光片，灵活 VersaMax读板机使用光栅单色器来选择 波长，其结果是选择确切的检测波长应用 于每个实验。波长范围从340nm到 With the VersaMax microplate reader, you will never have to buy another filter. 4. Conventions Used in this Manual. Plates were read using a microplate reader (VersaMax, Molecular Devices) at 595 nm. The Molecular Devices VersaMax ELISA Microplate Reader enables precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing consequence-free assay flexibility. Monday to Friday 7am - 5pm Pacific Time. VersaMax Absorbance Microplate Reader memungkinkan pemilihan hingga dua panjang gelombang sekaligus untuk deteksi absorbansi dalam. SoftMax® Pro Software Validation Package provides the most comprehensive documentation and tools available to validate GxP administrator features, software. Select Update Driver. 파장 정확도 : < ±0. The TBARS content was calculated according to the extinction coefficient as 155 mM −1 cm −1. The absorbance was then determined by an enzyme-linked immunosorbent assay (ELISA) reader (VERSAmax; Molecular Devices, Sunnyvale, CA) at a wavelength of 405 nm. Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0140 Rev. 00 Read more Molecular Devices Tunable Microplate Reader VersaMaxMicroplate reader with 450 nm filter, such as VersaMax (Molecular Devices, Sunnyvale, CA). After washing in PBS at least 4 times to remove all extracellular dye, samples were fixed using 4 % PFA/PBS for. The absorbance was measured at 450 nm using a microplate reader (VersaMax, Molecular Devices). ChE activity was calculated as the rate of change of absorbance per minute during the linear portion of the reaction. For more advanced automation needs, Molecular Devices interacts with all of the major lab automation providers, and is one of their leading choices. The samples were incubated with FBS, and the optical density of particles was determined with a microplate reader (VERSAmax, Molecular Devices, Sunnyvale, CA) by measuring the absorbance at 560 nm for 2 h. This manual is subject to change without notice. 8. Cell viability was quantified by microplate reader (VersaMax, Molecular Devices, San Jose, CA, USA) at 570 nm. 5 mM. Regular price $ 0. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. NORTH AMERICA. Microplate Reader Shelf. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial. Regular price $ 0. Flexible calculations Data analysis features are available such as 21 different curve fit options, simplified cross-plate analysis and interpolation, and automatic calculations. To evaluate the reliability of the assay, SkinEthic TM RHE (1% Triton X-100) was used. 8. The system provides automation for up to 50 For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. Our products enable scientists to improve productivity and efficiency in scientific discovery and research. spectrophotometer (VERSAmax, Molecular Devices). 2 and 648. TECHNICAL NOTE. This robust model allows precise wavelength detection in. JEG-3 cells were transfected with myc-tagged NDRG1 wild type or deletion mutants at the N- or C- terminal as depicted in Figure 5A. After waiting 15 minutes for colour development at room temperature, the reaction was stopped with 1 M H 2 SO 4, and optical density values were read using an ELISA reader (Versamax, Molecular Devices, LLC, USA) at a wavelength of 492 nm, and the results were given as OD values. Then, the cultured cells were treated with different. These values were transformed to logarithmic scale to create a log-log curve. 3860 N First Street San Jose,CA 95134 USA +1 408. The increase of absorbance at 405. Direct cytotoxicity of HVJ-E and Laserphyrin®-HVJ-EThe MTT formazan crystals were dissolved by adding 50 μl DMSO to each well and the plates gently shaken until the MTT solution was dissolved. To make your request, nothing more simple: you just have to connect on the site and to make your request on line. The Molecular Devices VersaMax ELISA Microplate Reader enables precise and accurate detection at. Cell proliferation. A. 6 nm, respectively, by ELISA microplate reader (VERSAmax, Molecular Devices, USA). HT22 cells are derived from hippocampal neuronal HT4 cells. The sections were stained with hematoxylin and eosin (H&E) and. The conversion of L-DOPA to dopachrome was allowed to proceed for 40 min at 30 °C in a microplate reader (Versamax; Molecular Devices). VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. 00 Regular price. Cell cycle investigation. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. It also integrates with the. Molecular Devices Beijing Tel. FRAP solutions were prepared as described previously (Benzie and Strain, 1996, Benzie and Strain, 1999). Select Start>Control panel>Device Manager. 0%, 0-2. Spectrophotometers. Color intensities were measured using microplate reader (Versamax, Molecular Devices, Inc. , Sunnyvale, CA). ISM Equipment Classification (Group 1, Class A) This equipment is designated as. The VersaMax Absorbance Microplate Reader allows for selection of. The assay procedure was in accordance with the manufacturer's instructions, and the results were analyzed with a microplate reader (VersaMax; Molecular Devices Ltd. The small footprint and touchscreen control allows for easy laboratory setup with minimal investment of time, cost, and effort. We would like to show you a description here but the site won’t allow us. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. 5×10 3 cells were seeded in 96-well plates, treated with indicated inhibitors for 48 or 72 hours, analyzed 16 hours after BrdU addition, and absorbance measured at 450nm using a spectrophotometer plate reader (VersaMax, Molecular. The cells were treated with 15, 25, 30, and 45 nm-particles in order to compare the cytotoxicities of different sized nanoparticles on the treated cells. The Molecular Devices VersaMax Microplate Reader is a high-performance laboratory instrument designed for fast and accurate measurements of absorbance, fluorescence, and luminescence from 6 to 384-well microplates. 제조사 : Molecular Devices. ® The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. 5°C @ 37°C Well-to. 0. Table of Contents. Technical Specifications. Study conductThe amounts of chlorophyll a and chlorophyll b were measured at 664. Lihat Video. The absorbance at wavelength 450 nm was detected using a micro-plate reader (VersaMax, Molecular Devices, USA). 45% of MTT reagent in cell culture media was substituted and incubated for 4 h before adding 100 μL of 100× DMSO. The. ¨ Molecular Devices is a life-science technology innovator Our integrated hardware and software solutions enable automated workflows—from start to finish—for any research application. For more sample throughput, the system can be easily integrated into a full robotic The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Get pricing and purchase lab equipment and instrumentation today. Read-out was performed within 30 minutes with a microtiter plate reader at 450 nm (Versamax, Molecular Devices). TECHNICAL NOTE. 23,225, Thermo Fisher Scientific). . The colorimetric reaction was read using an automated ELISA microplate reader (Versamax, Molecular Devices, USA) at 492 nm. molecular devices | 1311 orleans drive | sunnyvale, cA 94089 usA | email: info@moldev. 2. S. Each sample has a discrete sample beam and reference beam so that each well is measured directly. ). After incubation, the absorbance of the plate was measured at 450 nm using a microplate reader (VERSAmax™, Molecular Devices Corp. Statistical analysisThe absorbance was measured using a microplate reader (VERSAmax; Molecular Devices, San Jose, CA, USA) at an excitation wavelength of 530–560 nm. The signal produced was proportional to the amount of analyte present and interpolated from the calibration curve presented on each plate. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Sale Sold out. Product Overview VersaMax™ ELISA Microplate Reader Manufacturer Molecular Devices® | Available Worldwide Be the first to review this product Request Pricing Molecular Devices® Manufacturer Website. The absorbance was measured at 490 nm using a VersaMax ELISA reader (Molecular Devices, Sunnyvale, California, United States). Whether bacteria were partially or completely inactivated by bone cement. SoftMax® Pro Software is the leading solution for microplate reader control and data analysis, where our exclusive INSIDER members help to ensure it remains the most innovative research tool available. This technical note is designed to provide information . The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility offered by monochromator-based systems. ~ Bio-Tek ELx808 Microplate Reader $2,795. Wavelength Bandwidth °¤ 2. 4 Microplate Reader Software w WARRANTY. Home - University of Victoriaimported directly into Excel (Microsoft, Redmond, WA) from the ELISA microplate reader (VersaMax; Molecular Devices, Sunnyvale, CA, USA). reader (VersaMax, Molecular Devices, Sunnyvale, CA, USA) at 560 nm with background subtraction at 630 nm, based on which the corresponding cell viability was assessed. Cell. +86-21-6887-8820 fax +86-21-6887-8890 Germany Molecular Devices GmbH Tel. StakMax Microplate Handling System. Taking into account the price differences between each seller and each country. (JC-1) (Molecular Probes), respectively. 0A Sunnyvale, CA Made in USAVersaMax price. Windows Home Editions are not supported. The optical density of each well at 490 nm was then measured using a microplate spectrophotometer system (VersaMax; Molecular Devices, Sunnyvale, CA, USA). New and used Molecular Devices VersaMax's for sale on LabX. ® ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. Absor-bance of the solution was measured using a micro plate Reader (VersaMax, Molecular Devices) at 570 nm. After freeze-drying, the. Cell proliferation assay MDA-MB-231 cells were seeded at a density. StakMax Microplate Handling System. The robot-compatible VersaMax Reader is cost-effective—users never have to buy filters. Molecular Devices VersaMax Tunable Microplate Reader. Am J Respir Crit Care Med 1998; 157: 351–370. After freeze-drying, the. Louis, USA) was used to. 2 mM PLP and 5. New and used Molecular Devices VersaMax's for sale on LabX. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Whether its cell line development, 3D biology, drug screening—or a combination of the three—our technology maximizes throughput and reproducibility to give. The plate was read at 570 nm after 5 min using Versamax (molecular devices, United States). The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340–850nm). EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Coat plate with 100 μL each of appropriately diluted standards (Table 1),. It covers everything from installation, configuration, data acquisition, data analysis, curve fitting, to reporting and troubleshooting. ® SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 6 nm, respectively, by ELISA microplate reader (VERSAmax, Molecular Devices, USA). It provides both rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. , Spectramax Gemini; Molecular Devices) Tissue culture plates (24- and 96-well) Vacuum suction pumpRelated products. Finally, 0. 1 N NaOH (50 μl) followed by absorbance measurement at 405 nm with a plate reader (VersaMax, Molecular Devices, Sunnyvale, CA). Cell viability was represented by percentage values compared to a. 0 out of 5 $ 2,999. The mouse hippocampal neuronal cell line HT22 is frequently used as an in vitro model to investigate the role of hippocampal cholinergic neurons in cognitive functions. FAST AND ACCURATE METERTECH Microplate Reader $3,650. We would like to show you a description here but the site won’t allow us. 0 N sulfuric acid. Molecular Devices Versamax Absorbance Microplate Reader BN 02970. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Molecular Devices also offers a mass spectrometry-based solution for food safety testingWe would like to show you a description here but the site won’t allow us. 00. 1 software, the leading microplate data acquisition and analysis software, you can download this PDF file from Molecular Devices. g. A. to 11. Get pricing and purchase lab equipment and instrumentation today. Additionally, the VersaMax Microplate Reader can be seamlessly integrated with the StakMax® Microlate Handling System through the SoftMax Pro Software. 68 $. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. 00. The percentage of cytotoxicity was calculated using the following formula: cytotoxicity (%) = [1 − (At/As) × 100], where At and As refer to the absorbance of a tested. All cell lines were cultivated for 24 h in 96-well cell culture plates with an initial concentration of 1×10 5 cells/mL. 2. Briefly, at 3 h before each of the desired time points, 10 µl of the MTS reagent. The average survival rates for cells exposed to dry. The Molecular Devices VersaMax Absorbance Microplate Reader enables both precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing consequence-free assay flexibility. The RT-qPCR analysis was performed on a 7500 Fast Real-Time PCR system (Applied Biosystems, USA). SoftMax® Pro 7. They were then pre-treated with indicated concentration of drugs for 1 h prior to being treated or not with 5-HT or serum. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. SpectraMax Plus384, 190, 340PC384, and VersaMax Installation Guide Author: Molecular Devices Subject: Installation Guide Keywords: SpectraMax Versa and others Detection Platform Created Date: 5/17/2023 2:14:27 PM At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. 5 mM EDTA, 0. The mixture was incubate at 37 °C for 30 min, and then the reaction was stopped by the addition of 0. Cells were treated with 150 μl of DMSO and absorbance was quantified in 540 nm using the microplate spectrophotometer system (VersaMax, Molecular Devices, Sunnyvale, CA, USA). Finally, the optical density of the wells was determined at an excitation wavelength of 450 nm and a reference wavelength of 540 nm on a VersaMax (Molecular Devices) microplate reader. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. 2. Then, the absorbance at a wavelength of 450 nm was measured using an absorption microplate reader (VersaMax, Molecular Devices, USA) to evaluate the cell survival rate. The Indocyanine green (ICG, Sigma-Aldrich) assay measures the liver depuration capacity . 0200-6117. The optical density (OD) was measured at 570 nm (with a reference at 690 nm) by UV-visible spectrophotometer (VersaMax, Molecular Devices, CA, USA) in triplicates. Furthermore, VersaMax is reliable, which means more uptime and lower long-term cost of. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. Use Windows Search to search for “Add or Remove”. Products : General, Microplate Readers. The samples were incubated with FBS, and the optical density of particles was determined with a microplate reader (VERSAmax, Molecular Devices, Sunnyvale, CA) by measuring the absorbance at 560 nm for 2 h. S. SoftMax® Pro Software is the leading solution for microplate reader control and data analysis, where our exclusive INSIDER members help to ensure it remains the most innovative research tool available. It features a dual-monochromator system, a large dynamic range, and a high-throughput capability. HT22 cells are derived from hippocampal neuronal HT4 cells. VERSA MAX-LUNABLE, MOLECULAR DEVICES, MICROPLATE R. Software (optional), such as Softmax Pro (Molecular Devices). After 72-h of incubation, CellTiter 96 Aqueous One Solution was added and cells were incubated for a further 2 h. Table 1 Conditions for biofilm formation on lids of 96-pin microtiter plates. The plate was read at 570 nm using an ELISA reader (VERSAMax, Molecular Devices, VWR, Philadelphia, PA, USA). Monochromator, tunable in 1-nm increments. Optical Densitiy (OD) values were processed with a data reduction software (SoftMax Pro, Molecular Devices) using a 4-parameter logistic fitting algorithm to the standard curve, allowing the determination of antibody. +44-118-944-8000. Then the absorbance at 570 nm was measured using a microplate reader (VersaMax, Molecular Devices, San Jose, CA, USA). The cells were seeded on the bottom of 24-well plates with sterile glass coverslips. VersaMax ELISA Microplate Reader from Molecular Devices, LLC Description Enables precise and accurate detection at wavelengths in the visible spectrum without the use of. 0 out of 5 $ 2,999. Molecular Devices VersaMax Microplate Reader Molecular Devices VersaMax Microplate Reader Specifications. At 48 h after transfection the cells were exposed to CoCl 2 (200 µM) or vehicle. Activity was calculated as picomoles of substrate hydrolyzed per min. Molecular Devices公司的VersaMax读 板机为光栅型单色器读板机，拥有灵活检 测优势，无需加配额外的滤光片。 可调波长，无需滤光片，灵活 VersaMax读板机使用光栅单色器来选择 波长，其结果是选择确切的检测波长应用 于每个实验。波长范围从340nm到With the VersaMax microplate reader, you will never have to buy another filter. Concluding Remarks. The cellular viability. Turn the reader on and secure the USB cable. The. 003 OD ±1. The blue formazan concentration formed in viable cells was measured at 570 nm using spectrophotometer (Versamax, Molecular Devices, São Paulo, Brazil). from Molecular Devices Corporation. 747. ¨ We would like to show you a description here but the site won’t allow us. While the cell covers about. Add to Cart. and California VERSAMAX Tunable Microplate Reader 100-240V - 4A 50-60 HZ T4. SoftMax® Pro Software is the most published microplate reader control and data analysis software in the industry. printed in u. The percentages of inhibition of cell viability were calculated as the ratio between the absorbance of the compound-treated cells and cells with only the vehicle. Furnace Manuals. The. GABase was a mixture of γ-aminobutyrate glutamate amino transferase. used. With optimized reagents, option to operate the. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Manual for Molecular Devices versamax Laboratory Equipment (47 pages) Specifications: 1379/1379020-versamax. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 专为科研检测平台量身打造的灵活且强大的小型微孔板读板机（酶标仪），具备终点法和动力学检测模式，标准配置6块滤光片，覆盖蛋白定量、细胞活力、农药残留、ELISA实验等. 1% (w/v) naphtylethylenediamine-HCl], incubated at room temperature for 10 min, and then the absorbance at 540 nm was measured in a microplate reader (VersaMax™, Molecular. The absorbance was detected at 517 nm wavelength using a microplate reader (VersaMax, Molecular devices, USA). All the experiments were conducted at. The Molecular Devices VersaMax Absorbance Microplate Reader enables both precise and accurate detection at wavelengths in the visible spectrum. (VersaMax, Molecular Devices, Sunnyvale, CA, US). Add to Cart. The signal was developed using 3,3′, 5,5′-tetramethylbenzidine after streptavidin–peroxidase treatment. Phosphodiesterase isozymes: molecular targets for novel antiasthma agents. Page 6 SpectraMax Plus384, 190, 340PC384, and VersaMax. 4. Figure S2: The cellular localization of NDRG1 is not affected by N- or C- terminal deleted NDRG1 mutants under hypoxic conditions. Find Fanuc, Molecular Devices, and GE for sale on Machinio. Biosystems CytoFluor 4000 Multi Well Plate Reader $ 1,995. Next, 100 μL of a reagent was added to an equal volume of cell culture medium present in each well. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Electrolyte leakage analysis was conducted using 8-day-old rice seedlings before and after cold stress treatments in different time points (0, 6, and 11 days) at 4°C. Molecular Devices also offers a mass spectrometry-based solution for food safety testing We would like to show you a description here but the site won’t allow us. Select Start>Control panel>Device Manager. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. FAST AND ACCURATE METERTECH Microplate Reader $3,650. Filterless. VERSAMAX. 17. Tristar 3 Multimode Microplate Reader. Access all the resources, tools and information you need to stay productive. It features a modular design that allows users to add or change detection cartridges according to their needs. StakMax. US HEADQUARTERS. Find regional support. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. Whether its cell line development, 3D biology, drug screening—or a combination of the three—our technology maximizes throughput and reproducibility to give. The absorbance was detected at 570 nm with a Microplate Reader (VersaMax, Molecular Devices, Sunnyvale, CA). One microgram of RNA was. With its advanced features and user-friendly interface, the VersaMax is the perfect choice for a wide range of research. 1 M sodium phosphate buffer (pH 6. No accessories are needed and SoftMax® Pro Software. Turn the reader on and secure the USB cable. S. . . Monday to Friday 8am - 5pm GMT. Tested w/warranty. Sponsored. In general terms, plasma was added to a 96-well plate containing the targeted antisera, after rigorous washing steps and conjugation with a secondary detection antisera, and the resultant colorimetric reaction was read by scanning spectrophotometry (VersaMax, Molecular Devices, Sunnyvale, CA). Absolute values were attained with a standard curve composed of type I bovine skin collagen solution (0. At All-usermanuals. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. Table of Contents. and California) Tunable Microplate Reader 100-240V ~ 4A 50-60 HZ T4. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility offered by monochrome-based systems. It features a modular design that allows users to add or change detection cartridges according to their needs. Find regional support. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 10. To update the driver. BMG Labtech - THERMOstar. The reaction in the wells was stopped by the addition of 50 μl of 1 M sulfuric acid, which turned the wells yellow. The culture supernatant of cells (25 μl) was mixed with 225 μl of DMMB dye solution and the absorbance of solution mixture was measured at 525 nm with a spectorophotometer (VERSAmax; Molecular Devices Japan KK, Tokyo, Japan). The growth inhibitory rate (GIR) of treated cells was calculated by Equation 1. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Molecular Devices, LLC. The rate of cell viability was calculated using the following formula:The conversion of NADP+ to NADPH was measured as absorbance (340 nm) on a microplate reader (VersaMax, Molecular Devices Co. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Dual monochromatic, three modes detection, UV Vis Abs, Fluor, and Lum, cuvette. VersaMax™ Tunable Microplate Reader, Molecular Devices. The activity was measured at 40 °C following o-nitrophenol (ONP) release at 405 nm using a microplate reader (Versamax, Molecular Devices, San Jose, CA, USA). This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. 00 Read more Molecular Devices Tunable Microplate Reader VersaMaxMicroplate reader with 450 nm filter, such as VersaMax (Molecular Devices, Sunnyvale, CA). The absorbance was read with a multiwall spectrophotometer (VERSAmax, Molecular Devices, Sunnyvale, CA, USA) at 450 nm. SpectraMax and VersaMax Plate Readers Operator s Manual -- 0112-0126 Rev A Molecular De. Microplate Reader Shelf. Sale. Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. Cell viability was expressed as percent survival of untreated cells. Assays were conducted in a 96-well microplate using ELISA microplate reader (VersaMax Molecular Devices, USA) and absorbance was measured at 475 nm. Table of Contents. Full size table. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Cell proliferation assay MDA-MB-231 cells were seeded at a density. Culture medium was removed, and lysis buffer (10 ml) was pipetted into each well. The absorbance was measured at 570 nm and. SpectraMax® i3 Multi-Mode Detection Platform. 00 USDLocation: Hampton, New Hampshire, United States Member Since: 28th February, 2012 (12 years ago) Current Listings: 3982 Return Policy: WE OFFER 180 DAY WARRANTY ON ALL ITEMS. $1,195. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. 8 in. The calibration curve was fitted to a sigmoid by the four-parameter method in the SoftMax Pro software (Molecular Devices), and allowed the calculation of the concentration of native Antigen 18A. HUVECs were seeded into 96-well plates at a density of 30,000 cells/well and allowed to attach for 12 h. In the Apps and Features window, locate and uninstall SoftMax Pro.